PgNase F is an enzyme that is commonly used in molecular biology to digest glycoproteins. The full name of the enzyme is Peptide-N-glycosidase F, and it is produced by various strains of bacteria. PgNase F cleaves the bond between the N-glycosylated asparagine residue and the protein backbone, thereby releasing the glycan moiety from the protein. This enzyme is particularly useful for studying the role of glycosylation in protein structure and function. It is also commonly used to prepare samples for mass spectrometry-based analysis, as the removal of glycosylation can simplify protein identification. PgNase F is available commercially from a number of vendors, and it is typically supplied as a lyophilized powder.
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